Spettroscopia UV e visibile

SHIMADZU UV 1800

Visibile ed ultravioletto ad alta risoluzione. Analisi acque, terreni, rifiuti, analisi degli oli, sudio delle cinetiche di reazione.

Photometric Mode Measures the absorbance or transmittance at a single wavelength or at multiple (up to eight) wavelengths. In multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths. Spectrum Mode Obtains sample spectra using wavelength scanning. Changes in the sample can be tracked using repeated scans. Data processing operations, such as the enlargement or reduction of the spectra obtained, peak detection, and area calculations, are also possible Quantitation Mode Generates a calibration curve from standard samples, and uses it to calculate the concentrations of unknown samples. Various combinations of wavelengths (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order regression calibration curves) are possible. Kinetics Mode Measures the change in absorbance as a function of time, and thereby obtains enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected. Using this mode in combination with an MMC-1600/1600C multi-cell holder (8/16 series multi-cell) or a CPS-240A cell positioner (6 cells) enables the consecutive analysis of multiple samples. Time-Scan Mode Measures the change in absorbance, transmittance, or energy as a function of time. Using this mode in combination with an MMC-1600/1600C multi-cell holder (8/16 cells) or a CPS-240A cell positioner (6 cells) enables the simultaneous analysis of multiple samples. Multi-Component Quantitation Mode Separately quantitates up to eight components mixed in a single sample. Pure components or mixtures of components can be used as standard samples. Biomethod Mode (provided as standard) Capable of analyzing DNA and proteins using the following quantitation methods. • Nucleic Acid Quantitation- Quantitates DNA or protein using the absorbance at 260/230 nm or 260/280 nm. • Protein Quantitation • Lowry method • BCA method (using bicinchoninic acid) • CBB method (using Coomassie Brilliant Blue G-250) • Biuret method • UV absorption method (direct measurement at 280 nm)